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Expression of the immunotoxin increases the intracellular Kar2p level. The P. pastoris strains and conditions for protein expression and SDS-PAGE used were the same as those described in the legend to Fig. 4. Cell extracts of 4-h protein-expressing cultures were analyzed by Western blotting using anti-Kar2p as the primary antibody. (A) Samples of strains expressing immunotoxin under PGAP control. (B) Samples of strains expressing immunotoxin and other proteins under PAOX1 control.
