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Substrate specificity of the engineered enzymesa
| Enzyme | % Enzyme activity
|
|||
|---|---|---|---|---|
| GL-7ACA | GL-6APA | AD-6APA | CPC | |
| C130 | 100 | 90.6 | 58.6 | 2.3 |
| Y151αF | 114 | 99.8 | 70.6 | 2.3 |
| Q50βN | 142 | 130 | 87.2 | 2.5 |
| Y151αF/Q50βN | 106 | 97.0 | 66.3 | 2.3 |
The enzyme activity of wild-type CA130 toward GL-7ACA was used as a control. Data are expressed as the percentage of enzyme activity relative to the control.
