FIG. 1.

In vitro proliferative effects of HRX-ENL mutants. HRX-ENL chimeric proteins are shown schematically, with filled boxes denoting the three AT hook motifs homologous to HMG-I(Y) proteins and the BCB domain that shows similarity with mammalian DNMT. Stippled boxes indicate amino- and carboxy-terminal regions of ENL that show homology with AF9 and more distantly with yeast ANC1. An amino-terminal FLAG epitope tag is depicted by a filled circle. Gaps indicate deletions. Numbers at the top indicate amino acids at fusion sites in HRX and ENL, respectively. The proliferative capacity of hematopoietic progenitors transduced with different constructs was measured in a myeloid methylcellulose assay. The bar graph represents the number of colonies per 10⁴ input cells in a third round of serial methylcellulose plating (mean ± standard error of the mean, n ≥ 3).