FIG. 2.

Analysis of mutant HRX-ENL protein expression levels in transfected Bosc23 cells. Nuclear proteins (40 to 60 μg per lane) isolated from cells transiently transfected with the indicated constructs were subjected to Western blot analysis using either a monoclonal anti-FLAG (specificity M2) antibody (A), a polyclonal antiserum against ENL (B), or a monoclonal anti-HRX antibody (HRX107) (C and D). Asterisks indicate endogenous ENL (B) or breakdown products (7) from endogenous HRX (C and D). The positions of molecular mass markers are indicated.