FIG. 4.

DNA binding activity of the methyltransferase homology (BCB) region demonstrated by Southwestern blot analysis. The GST-BCB fusion protein (1, 0.5, and 0.25 μg) was spotted onto nitrocellulose membranes in parallel with similar amounts of GST as a control. The membranes were incubated with the indicated radiolabeled DNA probes, and bound DNAs were detected by autoradiography (left). To ensure that comparable amounts of test and control proteins were applied, GST-immunoreactive proteins were detected on the same membranes by Western blotting with an anti-GST monoclonal antibody (right).