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Southern hybridization analysis. The DNA probes AB1 (2.1 kb) and AB2 (1.5 kb) (see Fig. 1) were generated by PCR, labeled with DIG, and hybridized separately to the EcoRV-digested Anabaena sp. strain L-31 chromosomal DNA (3 μg) on Southern blots. The hybridization signals obtained with the two probes and their respective sizes are shown.
