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. 2005 Sep;71(9):5297–5303. doi: 10.1128/AEM.71.9.5297-5303.2005

FIG. 5.

FIG. 5.

Effect of osmotic stress on Anabaena sp. strain L-31 kdp expression. (A) Anabaena sp. strain L-31 cells were inoculated into BG-11/K0.05 medium containing different osmolytes as indicated. Total RNA (4 μg each) was hybridized to the kdpA2 probe. (B) Combined effect of extreme K+ limitation and NaCl stress on kdp2 expression. Anabaena sp. strain L-31 cells grown in BG-11/K5 were inoculated into BG-11/K0 with or without NaCl (65 mM) for the indicated time intervals. In another experiment, the cells grown in BG-11/K0 medium for 5 h were supplemented with NaCl (65 mM), and the culture was incubated for a further 16 h with NaCl (indicated by an asterisk). Total RNA was isolated and hybridized to the kdpA2 (upper panel) or kdpA1 (lower panel) probe.