FIG. 7.

Effect of coculture time on the survival of C. jejuni NCTC 11351 during disinfection. Cocultures with C. jejuni and A. castellanii (CCAP 1501/10) and T. pyriformis (CCAP 1630/14A) (1:1 ratio of Campylobacter to protozoa) were prepared in PAS solution and incubated for up to 24 h at 25°C. After 3, 6, 9, 12, and 24 h, a 1:1,000 dilution of Virudine (1-min contact time) was used to kill planktonic C. jejuni, followed by neutralization with STS, gravity filtration (0.8 μm), rinsing and resuspension in PAS, sonication (10 s at 40 W), and viable Campylobacter counts. The results are presented as average (performed in quadruplicate) viable Campylobacter recovered per ml of PAS, and error bars indicate the standard deviations.