FIG. 4.

Characteristics of glass coverslip flow cell biofilms of B. cenocepacia K56-2 pHKT2 (♦), K56-I2 pHKT1 (⋄), and K56-R2 pHKT2 (○) as assessed by deconvolution epifluorescence microscopy. A) Biovolume was defined as the average percent area of active pixels over the slices of the stack multiplied by the stack depth (micrometers). B) Stack depth was defined as the number of slices in the stack multiplied by the slice depth (micrometers). C) Substratum coverage was defined as the percent area of active pixels recorded in the slice at the coverslip surface. All data points represent the means ± standard errors of the means from at least three separate trials, with three image stacks captured per time point per trial.