tetX PCR amplification products of DNA extracted from isolated colonies of the C. tetani strains. Lane 1, strain A; lane 2, strain IM1116; lane 3, strain CN1339; lane 4, strain D11, plasmid-positive colony; lane 5, strain D11, plasmid-negative colony; lane 6, strain G761, plasmid-positive colony; lane 7, strain G761, plasmid-negative colony; lanes M, 1-kb plus DNA ladder PCR marker.