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. 2005 Sep;71(9):5604–5606. doi: 10.1128/AEM.71.9.5604-5606.2005

FIG. 3.

FIG. 3.

PFGE and Southern blot analysis of the selected colony types from strains CN1339 and G761. (A) PFGE of genomic DNA after SmaI or ApaI digestion. (B) Southern blotting following PFGE of genomic DNA fragments digested with SmaI or ApaI using a tetX-specific probe. Lane 1, clone CN1339-A; lane 2, clone CN1339-B; lane 3, plasmid-negative clone of the G761 strain; lane 4, plasmid-positive of the G761 strain; lanes M, DNA ladder PFGE marker. The black arrow indicates the position of the gel well, and the dotted arrow indicates the position of the plasmid (74 kb). Results are representative of three independent experiments.