FIG. 5.

MRCKα affects the organization of cellular structures. HeLa cells grown on coverslips were microinjected with a plasmid encoding HA-tagged wild-type MRCKα (a and b), kinase-dead MRCKα^K106A (c and d), or kinase domain alone (e to h). Two hours after incubation, cells were fixed and stained with anti-HA antibody (a and c) or doubly stained with phalloidin (b, d, and e) or antibodies against myosin light chain (f), vinculin (g), or tubulin (h). Arrows indicate the injected cells located by HA staining (not shown in panels e to h). Bar = 10 μm.