TABLE 2.
Strain or LSDa | Hatchingb | Mortalityb |
---|---|---|
Control | 83a | 18a |
CHA0 | 51b | 56b |
CHA89 | 77a | 25a |
CHA805 | 70a | 31a |
LSDc | 17 | 14 |
Strains CHA0 (wild type), CHA89 (gacA), and CHA805 (aprA′-′lacZ) were grown in 1/20 King's B medium for 48 h. The control consisted of sterile medium.
Hatching and mortality were tested as previously described (22) and are expressed as the percentage of eggs hatched after 72 h of exposure and as the percentage of juvenile mortality after 24 h of exposure, respectively. Data for each strain and medium were obtained from three different experiments with six repetitions. Means of the experiments in each column followed by different letters are significantly different (P ≤ 0.05) according to Fisher's test.
LSD, least significant difference.