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. 2005 Sep;71(9):5145–5153. doi: 10.1128/AEM.71.9.5145-5153.2005

FIG. 2.

FIG. 2.

Distribution of samples positive for Campylobacter DNA obtained from digesta (A and C) and tissues (B and D) of the intestinal tract of steer A. (A and B) Genus Campylobacter DNA examined with conventional PCR. The intensity of the genus amplicon was assessed based on a scale from 0 to 4 relative to a standard sample of known DNA. (C and D) Abundance of C. jejuni (genome copies in 2 μl of template) determined by nonnested real-time quantitative PCR targeting the mapA gene. The horizontal line with vertical lines in panel D indicates the various regions of the small and large intestines, where “a” is the duodenum, “b” is the jejunum, “c” is the ileum, “d” is the cecum, “e” is the ascending colon, “f” is the transverse colon, “g” is the descending colon, and “h” is the rectum. The arrow indicates a region where there was abundant C. jejuni DNA. The total length of the small and large intestines was 26.1 m.