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Relationships between hybridization temperature and average hybridization signal intensity of 96 ORFs selected from S. oneidensis MR-1. Five hundred nanograms of genomic DNA (A) or 10 μg of cDNA target (B) was labeled and hybridized with the PCR-oligonucleotide microarray at 42°C, 45°C, 50°C, and 60°C in the presence of 50% formamide for 16 h. The data are averages and standard deviations for three slides and a total of six possible spots.
