TABLE 1.
Numbers of detected ORFs out of 96 at an SNR of >3.0 by PCR and oligonucleotide probes when different amounts of genomic DNA from S. oneidensis MR-1 were labeled and hybridized with the PCR-oligonucleotide mixed array
| gDNA (ng) | pg/gene | No. of ORFs detecteda
|
|||||
|---|---|---|---|---|---|---|---|
| PCR | 70-mer | 60-mer | 50-mer | 40-mer | 30-mer | ||
| 0.2 | 0.04 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1.0 | 0.2 | 41 ± 3.3 | 0 | 0 | 0 | 0 | 0 |
| 5.0 | 1.0 | 95 ± 0.7 | 38 ± 4.7 | 8 ± 3.2 | 2.3 ± 1.3 | 0 | 0 |
| 25 | 5 | 95 ± 0.7 | 56 ± 4.3 | 14 ± 5.3 | 7.3 ± 3.4 | 0 | 0 |
| 100 | 20 | 95 ± 0.8 | 94 ± 0.7 | 66 ± 6.7 | 57 ± 4.5 | 0 | 0 |
| 500 | 100 | 96 ± 0.0 | 95 ± 0.4 | 86 ± 6.0 | 66.7 ± 6.7 | 2.3 ± 1.3 | 0 |
a
The data are averages ± standard deviations for three slides with a total of six spots.