TABLE 2.
Probe | Parameter | pH 4
|
pH 10
|
||
---|---|---|---|---|---|
30 min | 60 min | 30 min | 60 min | ||
PCR | Detected gene no. | 95 ± 0.7 | 92 ± 2.3 | 83 ± 2.5 | 89 ± 3.2 |
Induced gene no. | 35 ± 1.8 | 25 ± 2.7 | 2 ± 0.0 | 7 ± 01.2 | |
Repressed gene no. | 5 ± 0.5 | 5 ± 0.6 | 76 ± 4.9 | 32 ± 3.7 | |
70-mer | Detected gene no. | 89 ± 5.3 | 79 ± 4.3 | 48 ± 2.7 | 66 ± 3.1 |
Induced gene no. | 38 ± 3.2 | 21 ± 2.5 | 2 ± 0.3 | 8 ± 1.6 | |
Repressed gene no. | 6 ± 0.4 | 5 ± 0.6 | 42 ± 3.8 | 20 ± 2.1 | |
60-mer | Detected gene no. | 68 ± 5.4 | 65 ± 2.5 | 36 ± 3.0 | 52 ± 4.1 |
Induced gene no. | 39 ± 1.9 | 22 ± 3.7 | 0 | 10 ± 2.2 | |
Repressed gene no. | 3 ± 0.5 | 3 ± 0.5 | 33 ± 3.8 | 13 ± 2.5 | |
50-mer | Detected gene no. | 69 ± 3.3 | 54 ± 5.0 | 25 ± 3.2 | 38 ± 4.1 |
Induced gene no. | 38 ± 2.1 | 21 ± 2.8 | 1 ± 0.0 | 5 ± 1.6 | |
Repressed gene no. | 3 ± 0.5 | 2 ± 0.3 | 20 ± 2.3 | 13 ± 1.8 |
The data were determined by using PCR amplicon or oligonucleotide arrays and are averages ± standard deviations for three slides with a total of six spots.