TABLE 3.
ORFs and average fold changes in gene expression determined with PCR or oligonucleotide probes when S. oneidensis MR-1 cells were exposed to pH 4.0 for 30 mina
| Locus | Fold change
|
|||
|---|---|---|---|---|
| PCR | 70-mer | 60-mer | 50-mer | |
| SO0105 | 8.6 ± 1.77b | 10.0 ± 2.01 | 11.0 ± 1.96 | NAc |
| SO0017 | 7.5 ± 1.05 | 3.8 ± 0.32 | NA | 2.7 ± 0.30 |
| SO1383 | 5.7 ± 0.63 | 3.4 ± 0.29 | NA | 3.6 ± 0.42 |
| SO2743 | 4.8 ± 0.50 | 4.6 ± 0.67 | NA | 7.6 ± 0.68 |
| SO4180 | 4.4 ± 0.39 | 5.5 ± 0.73 | NA | NA |
| SO2320 | 4.2 ± 0.26 | NA | NA | NA |
| SO3051 | 4.2 ± 0.34 | 5.2 ± 0.45 | NA | 2.6 ± 0.31 |
| SO2697 | 3.7 ± 0.41 | 5.2 ± 0.61 | NA | 2.2 ± 0.29 |
| SO4661 | 3.6 ± 0.45 | 4.5 ± 0.51 | 7.5 ± 0.65 | NA |
| SO1679 | 3.6 ± 0.51 | 4.6 ± 0.37 | NA | 4.0 ± 0.49 |
| SO2389 | 3.5 ± 0.30 | 3.2 ± 0.36 | 5.7 ± 0.46 | NA |
| SO2953 | 3.5 ± 0.42 | 3.1 ± 0.37 | 5.8 ± 0.49 | NA |
| SO2113 | 3.0 ± 0.25 | 3.1 ± 0.34 | 5.9 ± 0.68 | NA |
| SO2654 | 2.5 ± 0.22 | 1.4 ± 0.21 | 5.2 ± 0.55 | NA |
| SO1157 | 2.2 ± 0.18 | 2.8 ± 0.25 | NA | 1.6 ± 0.27 |
| SO2930 | 2.1 ± 0.19 | 1.2 ± 0.35 | NA | NA |
| SO1277 | 0.4 ± 0.08 | NA | 1.0 ± 0.09 | 0.6 ± 0.05 |
| SO2886 | 0.2 ± 0.07 | 0.5 ± 0.08 | NA | 0.8 ± 0.22 |
a
All 18 ORFs were detected by the PCR probes, but at least one oligonucleotide probe failed to detect one of the genes at an SNR of >3.0.
b
The data are averages ± standard deviations for three slides with a total of six spots.
c
NA, data are not available because of empty spots, poor spots, or manually removed bad spots.