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. 2005 Sep;71(9):5066–5076. doi: 10.1128/AEM.71.9.5066-5076.2005

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FIG. 6. — (A) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the His₆-tagged ValA protein. Lanes: 1, molecular weight markers; 2, soluble protein of cell extract of E. coli BL21Gold(DE3)pLysS/valA before induction with IPTG; 3, total protein of cell extract of E. coli BL21Gold(DE3)pLysS/valA after induction with IPTG; 4, soluble protein of cell extract of E. coli BL21Gold(DE3)pLysS/valA after induction with IPTG; 5, purified His₆-tagged ValA. (B) Thin-layer chromatography analyses of ValA assay (solvent system, n-butanol:ethanol:H₂O, 9:7:4). Lane 1, ValA without substrate; lane 2, boiled ValA with sedoheptulose 7-phosphate; lane 3, ValA with sedoheptulose 7-phosphate; lane 4, 2-epi-5-epi-valiolone.