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. 2025 May 27;122(22):e2503570122. doi: 10.1073/pnas.2503570122

Fig. 7.

Fig. 7.

Action potential clamp determination of Nav1.8 and Nav1.7 components of current during repetitive firing. (A) The record of repetitive firing evoked by 1-s current injection was used as command waveform after switching the amplifier to voltage clamp mode and currents evoked by the waveform were recorded in control (black) and after application of 30 nM VX-548 (red). (B) Top, current during 1st, 2nd, and 5th (last) action potentials before and after application of VX-548. Middle, subtraction yielding Nav1.8 (VX-548-sensitive) current. Bottom, subtraction yielding Nav1.7 current by application of 30 nM GsAF-1 in the continuing presence of VX-548. (C) Collected results of peak current sensitive to 30 nM VX-548 during the 1st, 2nd, 3rd, 4th, and last action potentials in 10 neurons. Each cell used a command voltage from its own repetitive firing. (D) Collected results of peak current sensitive to 30 nM GsAF-1 (in the presence of VX-548) in these neurons.