FIG. 1.
Inhibition of intact E47 by cytoplasmic Notch1 and Notch2. NIH 3T3 cells were transfected with the expression plasmids for the indicated proteins and reporter [μE5+μE2]6TATA-CAT (100 ng; lanes 1 to 8) or [GAL4]5E1bTATA-CAT (250 ng; lanes 9 to 11). CMV-E47 was used to express full-length E47 (30 ng; lanes 2 to 4), CMV-E47-VP16 was used to express the E47-VP16 fusion protein (60 ng; lanes 6 to 8), and CMV-GAL4-E47 was used to express the GAL4-E47 fusion protein (100 ng; lanes 10 and 11). Either 1 (lanes 3 and 7) or 6 (lanes 4, 8, and 11) μg of N1-IC (N lanes in A) or N2-IC (N lanes in B) was included in the transfections, as indicated.