FIG. 2.

Notch1 does not inhibit CBP/p300. (A) E47 activity was determined in 293T cells transfected with the [μE5+μE2+μE3]₆TATA-luciferase reporter (100 ng) in the presence of either Notch1 (N1; 1 μg), p300 (5.5 μg), or both, as indicated. (B) GAL4-p300 fusion protein activity was determined in 293T cells transfected with the [GAL4]₅E1bTATA-luciferase reporter (300 ng) in the presence or absence of Notch1, as indicated. (C) Activity of RAR (0.5 μg) was determined by using the RAR-TK-luciferase reporter (2 μg) in the presence or absence of 1 μM retinoic acid (RA) and in the presence of RA plus Notch1 (1 μg), as indicated.