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Primers for construction and confirmation of deletion strains
| Primer namea | Sequence (5′-3′) |
|---|---|
| YJL-F | TGTTTAAAAGGGGAAGGTACGA |
| YJL-R | CGCGAAGAAAATATGGAATTA |
| YAL-F | ACGAGATTAGCGACCTCGAA |
| YAL-R | CGCATGCATATGCCTTTATG |
| YFR-F | CGATTTTATTGTCGCCTCTTG |
| YFR-R | TCATTTCCGCTGAATAAGTACG |
| KanMX4-R | CGACTGAATCCGGTGAGAAT |
F and R, forward and reverse primers, respectively.
