Growth of the insertion mutants on DDVA. Cells of SYK-6 (open diamonds), DW (open squares), DW2 (open triangles), DDW (open circles), DDW harboring pVKW2 (solid circles), and DDW harboring pVK7E2 (solid squares) were grown in W medium containing 5 mM DDVA at 30°C. The values are averages ± standard deviations (error bars) of three independent experiments.