FIG. 3.

High levels of Tim23Cp are toxic to tim23-1 cells. The tim23-1 strain KRR131 and the wild-type (WT) strain RJ500 were transformed with each of the following plasmids: GAL1-TIM23 (13), GAL1-TIM17 (57), and GAL1-tim23C (pKR17). Transformants were patched onto selective medium containing 2% raffinose as the sole carbon source. The patches were then replica plated onto selective medium containing 2% galactose to induce the expression of the plasmid-borne genes. All the cells were grown at 24°C, the permissive temperature for tim23-1.