FIG. 6.

A subset of E2F target genes is derepressed specifically in p130^−/−;p107^−/− peripheral T lymphocytes. Total RNA was purified from T-cell-enriched (85 to 90% pure) splenocytes of reconstituted animals and analyzed by Northern blotting (10 μg/lane). (Left and middle panels) Blots with T-lymphocyte RNA from chimeric and control wild-type animals, as well as cycling MEFs, were sequentially hybridized with the indicated probes. (Right panel) RNAs from the indicated lymphocytes and E1A-expressing MEFs were resolved and hybridized with the indicated probes. Acidic ribosomal phosphoprotein PO (ARPP-PO) was used as a loading control.