Table 2.

F-measure values for Cellpose-CPPA and CellProfiler cytoplasm segmentation of NAD(P)H images of quiescent T cells, activated T cells, and MCF7 cells. F-measure values are computed for each identified cell via POSEA. Values are reported as mean (± standard deviation), n = 982 quiescent T cells, 228 activated T cells, and 335 MCF7 cells. From the subsequent statistical analysis, it was found that the F-measure for the CellProfiler method is significantly lower (p < 2e-16) compared to the CPPA threshold methods. In addition, within the CPPA thresholding methods, no significant differences were observed except for the mean thresholding method applied to quiescent T cells, which showed a significantly lower F-measure (p < 2e-16) compared to other thresholding methods. This aligns with the analysis presented in Table 1.

Segmentation Algorithm	F-measure
	Quiescent T cells	Activated T cells	MCF7 cells
CellProfiler	0.59 (±0.23)	0.55 (±0.21)	0.46 (±0.29)
CPPA-isodata	0.83 (±0.09)	0.76 (±0.13)	0.72 (±0.21)
CPPA-li	0.83 (±0.09)	0.75 (±0.14)	0.72 (±0.21)
CPPA-mean	0.80 (±0.09)	0.75 (±0.14)	0.72 (±0.21)
CPPA-otsu	0.83 (±0.09)	0.76 (±0.14)	0.72 (±0.21)
CPPA-triangle	0.84 (±0.08)	0.76 (±0.14)	0.69 (±0.20)
CPPA-yen	0.83 (±0.09)	0.75 (±0.13)	0.72 (±0.21)