FIG. 10.

Effects of competitor phospho- and dephosphopeptides on the interaction between Ret and Enigma. Lysates from NIH 3T3 cells expressing the EGFR-Ret chimeric receptor were incubated with GST-LIM2 of Enigma bound to glutathione agarose beads. The incubation was carried out in the presence of various concentrations of peptide containing the last 20 residues of Ret/ptc2, residues 577 to 596. The peptide was synthesized to contain either phosphotyrosine 586 (pY586) or tyrosine 586 (Y586). After incubation the beads were washed extensively, and bound EGFR-Ret was visualized by Western blotting with anti-Ret antibody.