Appendix 1—table 11. Rates of sperm penetration and binding to murine ZP after homologous (murine sperm) and heterologous (human and bovine sperm) EZPT using bovine ZPs co-incubated without OVGP1 (HoA, HeAh, HeAb) or with in vivo-matured murine OVGP1 (oocytes from superovulated females) (HoBb, HeBh, HeBb), murine OVGP1 (HoC, HeCh, HeCb), human OVGP1 (HoD, HeDh, HeDb) or bovine OVGP1 (HoE, HeEh, HeEb).
| Group N | Sperm | OVGP1 | TMC | Penetrated ZPs (%) | Sperm penetration inside ZP (mean) | Max. no. of sperm inside a single ZP | ZP with bound sperm (%) | Sperm bound to ZPs (mean) | Max. no. of sperm bound to a single ZP |
|---|---|---|---|---|---|---|---|---|---|
| HoA 56 |
Murine | - | 8.76±1.08 | 56 (100±0)b |
848 (15.14±1.19)b |
29±5.50 | 56 (100±0) |
1042 (18.60±2.21) |
32±2.06 |
| HeAh 85 |
Human | - | 6.12±0.17 | 60 (70.83±5.89)c |
177 (2.95±0.07)c |
9±0.71 | 79 (92.92±0.59) |
263 (3.32±0.31)d |
11±1.41 |
| HeAb 97 |
Bovine | - | 3.67±0.19 | 56 (57.85±5.45)c |
167 (2.95±0.02)c |
6±0.00 | 91 (93.87±2.65) |
303 (3.32±0.40)d |
10±1.41 |
| HoB 19 |
Murine | Murine | 5.35±0.07 | 19 (100±0)b |
228 (12.00±0.15)b |
31±7.78 | 19 (100±0) |
313 (16.47±0.64)b |
34±2.83 |
| HeBh 30 |
Human | Murine | 6.12±0.17 | 1 (6.67±4.71)a |
1 (1±0)a |
1±0 | 3 (10.00±4.71) |
3 (1.00±0.00)a |
1±0 |
| HeBb 27 |
Bovine | Murine | 4.26±0.09 | 1 (6.25±4.42)a |
1 (1±0)a |
1±0 | 3 (10.80±2.41) |
3 (1.00±0.00)a |
1±0 |
| HoC 99 |
Murine | Human | 6.33±1.46 | 67 (67.49±2.39)c |
228 (3.40±0.24)c |
11±1.41 | 97 (98.00±2.82) |
449 (4.63±0.09)d |
19±0.71 |
| HeCh 104 |
Human | Human | 5.60±0.91 | 67 (64.39±2.32)c |
185 (2.76±0.02)c |
8±0.71 | 101 (10.38±0.54) |
1079 (10.68±0.54)c |
29±2.12 |
| HeCb 30 |
Bovine | Human | 6.09±1.31 | 0 (0±0)a |
0 (0±0)a |
0±0 | 2 (6.67±0) |
2 (1.00±0)a |
1±0 |
| HoD 88 |
Murine | Bovine | 5.29±0.43 | 56 (63.80±1.49)c |
219 (3.91±0.21)c |
8±0.71 | 87 (99.01±1.39) |
459 (5.28±0.62)d |
19±1.41 |
| HeDh 25 |
Human | Bovine | 5.45±0.75 | 0 (0±0)a |
0 (0±0)a |
0±0 | 1 (3.33±4.71) |
1 (1.00±0.04)a |
1±0.71 |
| HeDb 90 |
Bovine | Bovine | 4.35±0.46 | 54 (59.88±1.90)c |
182 (3.37±1.38)c |
7±0.71 | 90 (100±0) |
956 (10.62±0.08)c |
28±1.41 |
HoA = homologous EZPT with murine sperm. HeAh = heterologous EZPT with human sperm. HeAb = heterologous EZPT with bovine sperm. HoB = homologous EZPT with murine sperm (ZPs were co-incubated with murine OVGP1 for 30 min). HeBh = heterologous EZPT with human sperm (ZPs were co-incubated with murine OVGP1 for 30 min). HeBb = heterologous EZPT with bovine sperm (ZPs were co-incubated with murine OVGP1 for 30 min). HoC = homologous EZPT with murine sperm (ZPs were co-incubated with human OVGP1 for 30 min). HeCh = heterologous EZPT with human sperm (ZPs were co-incubated with human OVGP1). HeCb = heterologous EZPT with bovine sperm (ZPs were co-incubated with human OVGP1 for 30 min). HoD = homologous EZPT with murine sperm (ZPs were co-incubated with bovine OVGP1 for 30 min). HeDh = heterologous EZPT with human sperm (ZPs were co-incubated with bovine OVGP1). HeDb = heterologous EZPT with bovine sperm (ZPs were co incubated with bovine OVGP1 for 30 min). TMC = total motile sperm count after capacitation calculated by multiplying the volume by concentration (million sperm/mL) and by the motility (%) after capacitation. Penetrated ZP refers to the mean number of ZPs penetrated by at least one sperm. ZPs with bound sperm was calculated as the mean number of ZP with, at least, one bound sperm. Sperm penetration was taken as the total number of sperm observed inside the zonas. Bound sperm was expressed as the mean number of sperm that remained bound to the ZP. “N” refers to the total number of ZPs fertilized per treatment. Analysis was conducted by using a one-way ANOVA, followed by Tukey´s post hoc test. Different superscripts (a, b, c. d) in the same column indicate significant differences (p<0.05; n=3). Values are expressed as the mean ± SD.