Appendix 1—table 5. Rates of sperm penetration and binding to the murine ZP after the homologous (murine sperm) and heterologous (bovine sperm) EZPT using ZPs prepared from oocytes from murine ovaries (HoA, HeA) and from the oviducts of superovulated female mice (HoB, HeB).
| Group N | Sperm | ZPs from murine oocytes | TMC | Penetrated ZPs (%) | Sperm penetration inside ZP (mean) | Max. no. of sperm inside a single ZP | ZP with bound sperm (%) | Sperm bound to ZPs (mean) | Max. no. of sperm bound to a single ZP |
|---|---|---|---|---|---|---|---|---|---|
| HoA 56 | Murine | Ovary | 8.76±1.07 | 56 (100±0)a |
848 (15.08±1.19)a |
29 ± 5.50a | 56 (100±0)a |
1042 (19.02±2.21)a |
32 ± 2.06a |
| HeAb 97 | Bovine | Ovary | 3.67±0.19 | 56 (57.85±5.45)b |
167 (2.98±0.02)b |
10±1.4b | 91 (93.87±2.65)b |
303 (3.33±0.52)b |
9±1.42b |
| HeAh 85 | Human | Ovary | 6.12±0.17 | 60 (70.83±5.89)b |
177 (2.95±0,07)b |
9±0.71b | 79 (92.92±0.59)b |
263 (3.34±0.35)b |
11±1.41b |
| HoB 37 | Murine | Oviduct | 8.76±1.07 | 37 (100±0)a |
423 (23.86±0.79)a |
18 ± 4.24a | 37 (100±0)a |
645 (10.02±1.81)a |
28 ± 2.83a |
| HeBb 131 | Bovine | Oviduct | 3.67±0.19 | 3 (6.08±1.36)c |
3 (1±0)c |
1 ± 0c | 21 (21.46±2.06)c |
62 (2.96±0.33)c |
4 ± 0.71c |
| HeBh 65 | Human | Oviduct | 6.12±0.17 | 3 (4.72±0,39)c |
3 (0.05±0.01)c |
1 ± 0c | 14 (22.50±3.54)c |
39 (2.70±0.42)c |
5 ± 2.83c |
HoA = homologous EZPT with murine sperm (oocytes obtained from ovary). HeAb = heterologous EZPT with bovine sperm (oocytes obtained from ovary). HeAh = heterologous EZPT with human sperm (oocytes obtained from ovary). HoB = homologous EZPT with murine sperm (oocytes obtained from the oviduct of SOV females). HeBb = heterologous EZPT with bovine sperm (oocytes obtained from the oviduct of SOV females). HeBh = heterologous EZPT with human sperm (oocytes obtained from the oviduct of SOV females). TMC = total motile sperm count after capacitation calculated by multiplying the volume by concentration (million sperm/mL) and by the motility (%) after capacitation. Penetrated ZPs refers to the mean number of ZPs penetrated by at least one sperm. ZPs with bound sperm was calculated as the mean number of ZP with, at least, one bound sperm. Sperm penetration was taken as the total number of sperm observed inside the zonas. Bound sperm was expressed as the mean number of sperm that remained bound to the ZP. ‘N’ refers to the total number of ZPs fertilized per treatment. Analysis was conducted by using a one-way ANOVA, followed by Tukey´s post hoc test. Different superscripts (a, b, c) in the same column indicate significant differences (p<0.05; n=3). Values are expressed as the mean ± SD.