Appendix 1—table 9. Rates of sperm penetration and binding to bovine ZP after homologous (bovine sperm) and heterologous (murine sperm) EZPT using bovine ZPs co-incubated without OVGP1 (HoA, HeA) or with bovine OVGP1 (HoB, HeB).
Sperm was also previously co-incubated without OVGP1 or with bovine OVGP1 (HoAb*, HeAb*, HoBb*, HeBb*) or with murine OVGP1 (HeBm*).
| Group N | Sperm | ZPs OVGP1 | Sperm OVGP1 | TMC | Penetrated ZPs (%) | Sperm penetration inside ZP (mean) | Max. no. of sperm inside a single ZP | ZP with bound sperm (%) | Sperm bound to ZPs (mean) | Max. no. of sperm bound to a single ZP |
|---|---|---|---|---|---|---|---|---|---|---|
| HoA 15 |
Bovine | - | - | 5.74±1.64 | 15 (100±0)a |
272 (18.13±1.32)a |
32±2.08 | 15 (100±0) |
338 (22.53±2.08)a |
25±2.51 |
| HoAb* 61 |
Bovine | - | Bovine | 5.74±1.64 | 61 (100.0±10.00)a |
963 (15.80±1.18)a |
31±1.53 | 61 (100±0) |
1093 (17.97±3.01)a |
31±1.15 |
| HeA 60 |
Murine | - | - | 4.31±0.94 | 55 (91.67±8.15)b |
236 (4.24±0.52)b |
16±1.15 | 60 (100±0) |
338 (5.63±0.48)b |
21±1.00 |
| HeAb* 55 |
Murine | - | Bovine | 4.31±0.94 | 49 (88.24±11.76)b |
198 (4.11±0.51)b |
14±1.00 | 55 (100±0) |
276 (5.00±0.31)b |
18±2.65 |
| HeAm* 58 | Murine | - | Murine | 4.31±0.94 | 52 (89.65±9.75)b |
233 (4.02±0.66)b |
15±1.73 | 58 (100±0) |
294 (5.08±0.32)b |
22±0.58 |
| HoB 14 |
Bovine | Bovine | - | 5.74±1.64 | 14 (100±0)b |
232 (16.65±0.99)a |
33±1.52 | 14 (100±0) |
288 (20.88±3.88)a |
33±2.08 |
| HoBb* 59 |
Bovine | Bovine | Bovine | 5.74±1.64 | 59 (100±0)a |
906 (15.34±0.66)a |
29±1.00 | 59 (100±0) |
1001 (16.87±2.34)a |
32±1.52 |
| HeB 16 |
Murine | Bovine | - | 4.31±0.94 | 0 (0±0)c |
0 (0±0)c |
0±0 | 15 (94.44±9.62) |
78 (4.90±0.61)b |
8±0.57 |
| HeBb* 48 |
Murine | Bovine | Bovine | 4.31±0.94 | 0 (0±0)c |
0 (0±0)c |
0±0 | 41 (84.81±10.67) |
115 (2,39±0.16)c |
11±1.52 |
| HeBm* 51 |
Murine | Bovine | Murine | 4.31±0.94 | 2 (3.98±3.51)c |
2 (1±0)c |
1±0 | 51 (100±0) |
229 (4.48±0.57)b |
9±1 |
HoA = homologous EZPT with bovine sperm. HoAb*=homologous EZPT with bovine sperm (sperm was previously co-incubated with bovine OVGP1 for 30 min). HeA = heterologous EZPT with murine sperm. HeAb*=heterologous EZPT with murine sperm (sperm was previously co-incubated with bovine OVGP1 for 30 min). HeAm*=heterologous EZPT with murine sperm (sperm was previously co-incubated with murine OVGP1 for 30 min). HoB = homologous EZPT with bovine sperm (ZPs were co-incubated with bovine OVGP1 for 30 min). HoBb*=homologous EZPT with bovine sperm (ZPs were co-incubated with bovine OVGP1 for 30 min) (sperm was previously co-incubated with bovine OVGP1 for 30 min). HeB = heterologous EZPT with murine sperm (ZPs were co-incubated with bovine OVGP1 for 30 min). HeBb*=heterologous EZPT with murine sperm (ZPs were co-incubated with bovine OVGP1 for 30 min) (sperm was previously co-incubated with bovine OVGP1 for 30 min). HeBm*=heterologous EZPT with murine sperm (ZPs were co-incubated with bovine OVGP1 for 30 min) (sperm was previously co-incubated with murine OVGP1 for 30 min). TMC = total motile sperm count after capacitation calculated by multiplying the volume by concentration (million sperm/mL) and by the motility (%) after capacitation. Penetrated ZP refers to the mean number of ZPs penetrated by at least one sperm. ZPs with bound sperm was calculated as the mean number of ZP with, at least, one bound sperm. Sperm penetration was taken as the total number of sperm observed inside the zonas. Bound sperm was expressed as the mean number of sperm that remained bound to the ZP. ‘N’ refers to the total number of ZPs fertilized per treatment. Analysis was conducted by using a one-way ANOVA, followed by Tukey´s post hoc test. Different superscripts (a, b, c) in the same column indicate significant differences (p<0.05; n=3). Values are expressed as the mean ± SD.