FIG. 4.

Lambda exonuclease digestion of mre11-58 meiotic DNA. Genomic DNAs from meiotic cells were prepared when DSBs were fully formed and were treated with lambda exonuclease. After inactivating exonuclease, genomic DNAs were cut with PstI and subjected to Southern hybridization after agarose gel electrophoresis as shown in Fig. 3. A DNA fragment cut produced by PstI EcoRI double digestion was added before exonuclease treatment as an internal control. Lanes 1 to 4, rad50S DNA; lanes 5 to 8, mre11-58 DNA.