FIG. 8.

DSB formation at the HIS4LEU2 locus in mre11-58S. Horizontal arrows, major two sites of DSBs, called sites I and II. Genomic DNAs from mre11-58S (HTY1115) and mre11-58 (HTY703) were prepared from cells collected at 0 and 8 h (only 8 h for mre11-58) after entering meiosis, and Southern blotting was performed to detect fragments corresponding to the parent (12.6 kb), site I (3.7 kb), and site II (6.0 kb) as described in the legend to Fig. 3. Lanes 1 and 3, 0-h sample; lanes 2 and 4, 8-h sample. Lanes 1 and 2 and 3 and 4 are the results of independent clones of HTY1115. Lane 5, mre11-58 (HTY703).