FIG. 5.

Terminal-fragment variation in offspring and in testis DNA samples. In an experiment with different parents, DNA from samples of spleen (S) or testis (T; two different concentrations) from the father (individual XI) or the spleen (S) of the mother (individual XII) and of whole embryos (numbered 1 to 8) were digested with MseI-RsaI and hybridized to a mix of telomere repeat probe and lambda probe. Results of duplicate experiments with the parent DNA digests are shown; the mother’s DNA sample next to embryo 8 lane was only partially digested, as indicated by staining with ethidium bromide. The right panel shows HaeIII or RsaI digests of spleen and the more concentrated testis DNA sample from individual XI. The sizes of marker fragments (lanes λ) are indicated on the left in kilobases. Arrowheads indicate examples of the bands from the father’s spleen DNA that are clearly different in size from the testis sample DNA bands.