Lysis buffer

Reagent	Final concentration in the lysis buffer	Volume per well (μL)	Volume for 1536 wells (8 plates) + 21.5% dead volume (μL)
Nuclease-Free Water		1.94	3620
Poly-ethylene Glycol 8000 (40% solution)	6.7%	0.5	933
Triton X-100 (10% solution)	0.1%	0.03	56
ERCC 1:4e5	-	0.015	28
RNase Inhibitor	0.5 U/μL	0.04	75
dNTPs (10 mM/each)	0.67 mM/each	0.2	373
Protease (1.09 AU/mL)	27 mAU/mL	0.075	140
Total		2.80	5225