2× mock RT buffer (stored at −20°C for a maximum of two months)

Reagent	1 reaction	750 reactions
Poly-ethylene Glycol 8000 (40%)	0.5 μL	375 μL
Triton X-100 (10% solution)	0.03 μL	22.5 μL
Tris-HCl pH 8.3 1 M	0.1 μL	75 μL
NaCl 1 M	0.12 μL	90 μL
MgCl2 100 mM	0.1 μL	75 μL
GTP 100 mM	0.04 μL	30 μL
DTT 100 mM	0.32 μL	240 μL
Nuclease-free water	0.79 μL	592.5 μL