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| Reagent | 1 reaction |
|---|---|
| 2× KAPA HiFi HotStart ReadyMix | 5 μL |
| 2× mock RT buffer | 2 μL |
| Template gDNA (∼5 ng/μL) or cDNA (∼300 ng/μL) | 1 μL |
| Forward pre-amplification primer 4 μM | 1 μL |
| Reverse pre-amplification primer 4 μM | 1 μL |
