Nested genotyping primer testing reaction (bulk gDNA/cDNA)
| Reagent | 1 reaction |
|---|---|
| KAPA 2G Ready Mix | 3.125 μL |
| 5 μM dilution of nested primer pair | 0.375 μL |
| Template gDNA (∼5 ng/μL) or cDNA (∼300 ng/μL) | 1.5 μL |
| Nuclease-free water | 1.25 μL |
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