Figure S1. CTBP2 limits inflammatory gene expression and promotes cell cycle progression in macrophages.

(A) RNA-seq volcano plot of genes regulated by Ctbp1 knockdown compared with control siRNA in BMDMs after LPS treatment. Solid dots are significantly regulated genes (absolute fold change > 1.4 and P < 0.05) with colors according to legend. Bold numbers indicate the amount of differentially expressed genes. (B) RNA-seq volcano plot for the Ctbp2 knockdown (compare with (A)). (C) Gene ontology (GO) enrichment for “Biological Process” in CTBP2-activated genes (fold change < −1.4 and P < 0.05 after Ctbp2 knockdown in (B)). Color intensity indicates log₁₀-transformed Benjamini-Hochberg adjusted P-value. (D) MA plots for RNA-seq performed in BMDMs comparing LPS versus vehicle response in control (siControl, top), Ctbp1 knockdown (siCtbp1, middle), and Ctbp2 knockdown (siCtbp2, bottom). Log₂-transformed fold induction by LPS versus log₂ mean expression. Green and purple dots indicate significantly (absolute fold change > 1.4 and FDR < 0.1) up- and down-regulated genes, respectively. Numbers indicate the total amount of significantly regulated genes. (E) RT–qPCR for Il12b, Ctbp1, and Ctbp2 after Ctbp1 or Ctbp2 knockdown in BMDMs. Relative expression of the indicated gene over the housekeeper Rplp0 from n = 3 replicates, gray dots are individual data points, and error bars show SD. ***P < 0.001, ANOVA followed by Tukey’s test. (F) RT–qPCR experiments for IL12B, CTBP1, and CTBP2 after CTBP1 and/or CTBP2 knockdown in human macrophages. Relative expression over RPLP0 from n = 4; gray dots are individual data points, and error bars show SD. P-values are displayed above boxes, *P < 0.05, paired t test.