Hyaluronidase Inhibitory Activity of Polysaccharides Separated from a Fermented Beverage of Plant Extracts

. 2025 May 20;72(2):7202202. doi: 10.5458/jag.7202202

Table 1. Comparison of hyaluronidase inhibitory activity between crude separated and four separated fractions from Super Ohtaka^®, and λ-carrageenan.

Sample	Protein (%) (Lowry method)^a)	Carbohydrate (%) (Phenol-sulfuric acid)^b)	Yield^c) (mg)	Final concentration (mg/mL)	Inhibition ratio^d) (%)	IC₅₀ (µg/mL)
Crude separated			200	0.1	0
				0.2	21.1 ± 6.0
				0.4	37.8 ± 7.8
OEP1	2.5	71.2 ± 3.4	6.4^e)	1.0	0	-
OEP2	8.4^e)	86.4 ± 3.2^e)	5.5^e)	1.0	0	-
OEP3	7.4^e)	68.5 ± 4.1^e)	11.5^e)	1.0	56.6 ± 6.8	860
OEP3-1	60.4	36.2 ± 1.9	2.3	1.0	43.6 ± 5.4	1,240

λ-Carrageenan			-	0.1	78.7 ± 9.4

^a) BSA standard.

^b) Glucose standard.

^c) Yield was the value obtained from 1 L of fermented liquid of plant extract.

^d) Mean ± SD, (n = 3).

^e) Okada et al. [6].