FIG. 5.

C-terminal requirements for prosequence cleavage. Krp1 mRNA and mRNAs from truncated forms of Krp1 were translated in Xenopus egg extract for 1 h in the presence of [³H]leucine before being analyzed by SDS-PAGE (lanes 1 to 6). mRNAs from Krp1[W578*], Krp1[S590*], and Krp1[Y611*] (included to provide a convenient reference point between processed and unprocessed proteins) were also translated for 24 h before analysis (lanes 7 to 9). The positions of molecular weight markers (in thousands) are shown on the left.