TABLE 2.

Inhibitory properties of different prosequences in vivo^a

Prosequence	Internal site	Primary site	Doubling time
			Actual (h)b	Relative
Krp1[KR82][KR102][R102*]	Lys-Arg	Lys-Arg	4.12	1.00
Krp1[KA82][KR102][R102*]	Lys-Ala	Lys-Arg	5.55	1.35
Krp1[KR82][Y100*]	Lys-Arg	Absent	4.21	1.02
Krp1[KA82][Y100*]	Lys-Ala	Absent	4.13	1.00

^aPlasmids containing the indicated Krp1 prosequence constructs under the transcriptional control of the nmt1 promoter were transformed into S. pombe cells expressing wild-type Krp1 from its chromosomal allele. Transformants were cultured at 29°C in the absence of thiamine to induce expression from the nmt1 promoter. 

^bData are averages of five separate determinations.