| Chemicals, peptides, and recombinant proteins |
|
| BODIPY FL C16 |
Invitrogen |
Cat#D3821; CAS: 158757-82-5 |
| Bradford Reagent |
Sigma-Aldrich |
Cat#B6916 |
| Chloroform |
Sigma-Aldrich |
Cat#650498; CAS: 67-66-3 |
| Coomassie blue R 250 |
Fluka |
Cat#27816; CAS: 6104-59-2 |
| Cryoplast |
Biopack |
Cat#1204.05 |
| DEAE-Trisacryl M |
Sepracor |
Cat#250771; CAS: 97930-01-3 |
| DiI |
Sigma-Aldrich |
Cat#468495; CAS: 41085-99-8 |
| Dithiothreitol |
Millipore |
Cat#10197777001; CAS: 3483-12-3 |
| DMSO |
Millipore |
Cat#D2650; CAS: 67-68-5 |
| Na2EDTA |
Mallinckrodt |
Cat#4931; CAS: 60-00-4 |
| FluorSave Reagent |
Millipore |
Cat#345789 |
| Formaldehyde |
Sigma-Aldrich |
Cat#F8775; CAS: 50-00-0 |
| FITC |
Sigma-Aldrich |
Cat#F7250; CAS: 27072-45-3 |
| Hydrochloric acid |
Mallinckrodt |
Cat#7647-01-0; CAS: 7647-01-0 |
| Methanol |
Sigma-Aldrich |
Cat#34860; CAS: 67-56-1 |
| NaCl |
Millipore |
Cat#116224; CAS: 7647-14-5 |
| Potassium bromide |
Mallinckrodt |
Cat#0505; CAS: 7758-02-3 |
| Monobasic potassium phosphate |
Sigma-Aldrich |
Cat#P5655; CAS: 7778-77-0 |
| Protease inhibitor cocktail |
Sigma |
Cat#P2714-1BTL |
| Sepharose 6B |
Sigma |
Cat#6B-100 |
| Sodium phosphate dibasic |
Sigma-Aldrich |
Cat#S9763; CAS 7558-79-4 |
| Sucrose |
Mallinckrodt |
Cat#8360; CAS: 57-50-1 |
| Tris |
Bio Basic |
Cat#TB0194; CAS: 126-72-7 |
|
| Experimental models: Organisms/strains |
|
|
Dipetalogaster maxima (vitellogenic females) |
Supplied by CIBICI-CONICET-UNC (Argentina) |
RRID:NCBITaxon_72496 |
|
Rhodnius prolixus (vitellogenic females) |
Supplied by CIBICI-CONICET-UNC (Argentina) |
RRID:NCBITaxon_13249 |
|
Panstrongylus megistus (vitellogenic females) |
Supplied by CIBICI-CONICET-UNC (Argentina) |
RRID:NCBITaxon_65343 |
|
| Software and algorithms |
|
| Adobe Illustrator |
Adobe Systems Incorporated |
Released 2020. RRID:SCR_010279
|
| Adobe PhotoShop |
Adobe Systems Incorporated |
Released 2021. RRID:SCR_014199
|
| ImageJ (Fiji version) |
Schindelin et al.13
|
https://imagej.nih.gov/ij/ RRID:SCR_002285
|
|
| Other |
|
| Abbe refractometer |
WYA |
2WA-J |
| Amber glass vials |
N/A |
N/A |
| Amicon ultra centrifugal filter, 100 kDa MWCO |
Millipore |
Cat#UFC9100 |
| Carbon steel edge blade |
Treet |
N/A |
| Chromatography column |
Pharmacia Fine Chemicals |
N/A |
| Clear nail polish |
N/A |
N/A |
| Corning PYREX Reusable Petri dishes |
Fisher Scientific |
Cat#08-747A |
| Cover glass 24 × 30 mm; thickness: 0.12 mm to 0.16 mm |
Corning |
Cat#2975-243 |
| Cryostat |
Thermo Scientific |
Model: Shadow Cryotome E |
| Dressing tissue forceps |
Merck |
Cat#F4642 |
| Metabolic shaking incubator |
Vicking |
Model: DUBNOFF |
| Extra fine Bonn scissors |
FST |
Cat#14084-08 |
| Fiber optic low-noise illuminator |
Cole-Parmer 9741-50 |
Model: 41510-00 |
| Fraction collector |
LKB BROMMA |
Model: 2112 Redirac |
| Glass beads, acid-washed |
Sigma |
Cat#G1145 |
| Hamilton syringe (Microliter #701) |
Sigma-Aldrich |
Cat#HAM80075 |
| Gradient maker |
Hoefer |
Cat#10049309 |
| Jewelers forceps, Dumont No. 5 |
Merck |
Cat#F6521 |
| Laser scanning confocal microscopy |
Olympus |
Model: FV1200 |
| Magnetic Plate Stirrer |
Dauerhaft |
Model: Mag D-320 digital |
| Micro-ultracentrifuge tube |
Beckman Coulter |
Cat#343776 |
| Micro-ultracentrifuge |
Beckman Coulter |
Model: Optima MAX-XP |
| Microscope slides |
HDA |
Cat#HDAS001 |
| Positive charged microscope slides |
HDA |
Cat#HDAS001 |
| Microtome blades |
Feather |
Cat#A35 |
| Mini-PROTEAN 3 cell for polyacrylamide gel electrophoresis (PAGE) setup |
Bio-Rad |
Cat#165-3301 |
| Nitrogen gas stream setup |
N/A |
N/A |
| Orbital shaker |
Vicking |
Model: M-23 |
| Paper masking tape |
N/A |
N/A |
| PD-10 Desalting columns |
Cytiva |
Cat#GE17-0851-01 |
| pH meter |
Corning |
Model: 430 |
| PowerPac Basic Power Supply |
Bio-Rad |
Cat#164-5050 |
| Spectrophotometer |
Agilent |
Model: BioTek Synergy HTX |
| Standard cellulose dialysis tubing |
Fisher Scientific |
Cat#0867139 |
| Stereoscopic microscope |
Schonfeld Optik microscope |
Model: LBX |
| Tabletop centrifuge |
Eppendorf |
Model: 5810/5810R (EP022628168) |
| Ultracentrifuge tubes (standard polypropylene Quick-Seal tube) |
Beckman Coulter |
Cat#342414 |
| Zeba Spin desalting columns and plates, 40K MWCO, 75 μL–10 mL |
Thermo Scientific |
Cat#A57756 |