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. 2025 May 22;6(2):103840. doi: 10.1016/j.xpro.2025.103840
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

BODIPY FL C16 Invitrogen Cat#D3821; CAS: 158757-82-5
Bradford Reagent Sigma-Aldrich Cat#B6916
Chloroform Sigma-Aldrich Cat#650498; CAS: 67-66-3
Coomassie blue R 250 Fluka Cat#27816; CAS: 6104-59-2
Cryoplast Biopack Cat#1204.05
DEAE-Trisacryl M Sepracor Cat#250771; CAS: 97930-01-3
DiI Sigma-Aldrich Cat#468495; CAS: 41085-99-8
Dithiothreitol Millipore Cat#10197777001; CAS: 3483-12-3
DMSO Millipore Cat#D2650; CAS: 67-68-5
Na2EDTA Mallinckrodt Cat#4931; CAS: 60-00-4
FluorSave Reagent Millipore Cat#345789
Formaldehyde Sigma-Aldrich Cat#F8775; CAS: 50-00-0
FITC Sigma-Aldrich Cat#F7250; CAS: 27072-45-3
Hydrochloric acid Mallinckrodt Cat#7647-01-0; CAS: 7647-01-0
Methanol Sigma-Aldrich Cat#34860; CAS: 67-56-1
NaCl Millipore Cat#116224; CAS: 7647-14-5
Potassium bromide Mallinckrodt Cat#0505; CAS: 7758-02-3
Monobasic potassium phosphate Sigma-Aldrich Cat#P5655; CAS: 7778-77-0
Protease inhibitor cocktail Sigma Cat#P2714-1BTL
Sepharose 6B Sigma Cat#6B-100
Sodium phosphate dibasic Sigma-Aldrich Cat#S9763; CAS 7558-79-4
Sucrose Mallinckrodt Cat#8360; CAS: 57-50-1
Tris Bio Basic Cat#TB0194; CAS: 126-72-7

Experimental models: Organisms/strains

Dipetalogaster maxima (vitellogenic females) Supplied by CIBICI-CONICET-UNC (Argentina) RRID:NCBITaxon_72496
Rhodnius prolixus (vitellogenic females) Supplied by CIBICI-CONICET-UNC (Argentina) RRID:NCBITaxon_13249
Panstrongylus megistus (vitellogenic females) Supplied by CIBICI-CONICET-UNC (Argentina) RRID:NCBITaxon_65343

Software and algorithms

Adobe Illustrator Adobe Systems Incorporated Released 2020. RRID:SCR_010279
Adobe PhotoShop Adobe Systems Incorporated Released 2021. RRID:SCR_014199
ImageJ (Fiji version) Schindelin et al.13 https://imagej.nih.gov/ij/ RRID:SCR_002285

Other

Abbe refractometer WYA 2WA-J
Amber glass vials N/A N/A
Amicon ultra centrifugal filter, 100 kDa MWCO Millipore Cat#UFC9100
Carbon steel edge blade Treet N/A
Chromatography column Pharmacia Fine Chemicals N/A
Clear nail polish N/A N/A
Corning PYREX Reusable Petri dishes Fisher Scientific Cat#08-747A
Cover glass 24 × 30 mm; thickness: 0.12 mm to 0.16 mm Corning Cat#2975-243
Cryostat Thermo Scientific Model: Shadow Cryotome E
Dressing tissue forceps Merck Cat#F4642
Metabolic shaking incubator Vicking Model: DUBNOFF
Extra fine Bonn scissors FST Cat#14084-08
Fiber optic low-noise illuminator Cole-Parmer 9741-50 Model: 41510-00
Fraction collector LKB BROMMA Model: 2112 Redirac
Glass beads, acid-washed Sigma Cat#G1145
Hamilton syringe (Microliter #701) Sigma-Aldrich Cat#HAM80075
Gradient maker Hoefer Cat#10049309
Jewelers forceps, Dumont No. 5 Merck Cat#F6521
Laser scanning confocal microscopy Olympus Model: FV1200
Magnetic Plate Stirrer Dauerhaft Model: Mag D-320 digital
Micro-ultracentrifuge tube Beckman Coulter Cat#343776
Micro-ultracentrifuge Beckman Coulter Model: Optima MAX-XP
Microscope slides HDA Cat#HDAS001
Positive charged microscope slides HDA Cat#HDAS001
Microtome blades Feather Cat#A35
Mini-PROTEAN 3 cell for polyacrylamide gel electrophoresis (PAGE) setup Bio-Rad Cat#165-3301
Nitrogen gas stream setup N/A N/A
Orbital shaker Vicking Model: M-23
Paper masking tape N/A N/A
PD-10 Desalting columns Cytiva Cat#GE17-0851-01
pH meter Corning Model: 430
PowerPac Basic Power Supply Bio-Rad Cat#164-5050
Spectrophotometer Agilent Model: BioTek Synergy HTX
Standard cellulose dialysis tubing Fisher Scientific Cat#0867139
Stereoscopic microscope Schonfeld Optik microscope Model: LBX
Tabletop centrifuge Eppendorf Model: 5810/5810R (EP022628168)
Ultracentrifuge tubes (standard polypropylene Quick-Seal tube) Beckman Coulter Cat#342414
Zeba Spin desalting columns and plates, 40K MWCO, 75 μL–10 mL Thermo Scientific Cat#A57756