| Antibody |
guinea pig anti-tj polyclonal |
Gift from Dorothea Godt |
|
1:5000 dilution |
| Antibody |
Rabbit anti-tey polyclonal |
Gift from Angelike Stathopoulos |
|
1:200 dilution |
| Antibody |
Rat anti-c15 polyclonal |
Gift from Gerard Campbell |
|
1:1000 dilution |
| Antibody |
Chicken anti-GFP polyclonal |
Life Technologies |
A-10262 |
1:1000 dilution |
| Antibody |
Rabbit anti-GFP polyclonal |
Life Technologies |
A-11122 |
1:1000 dilution |
| Antibody |
Rabbit anti-Unc-4 polyclonal |
Lacin et al., 2014
|
A-10262 |
1:1000 dilution |
| Antibody |
Mouse anti-Acj6 monoclonal |
DSHB |
Acj6 |
1:100 dilution |
| Antibody |
Rat anti-CadN monoclonal |
DSHB |
DN-Ex #8 |
1:25 dilution |
| Antibody |
Mouse anti-Neuroglian monoclonal |
DSHB |
BP104 |
1:25 dilution |
| Antibody |
Goat anti-rabbit Alexa Fluor 488 |
Life Technologies |
A-11034 |
1:500 dilution |
| Antibody |
Goat anti-rabbit Alexa Fluor 568 |
Life Technologies |
A-11011 |
1:500 dilution |
| Antibody |
Goat anti-rabbit Alexa Fluor 633 |
Life Technologies |
A-21070 |
1:500 dilution |
| Antibody |
Goat anti-rat Alexa Fluor 633 |
Life Technologies |
A-21094 |
1:500 dilution |
| Antibody |
Goat anti-chicken Alexa Fluor 488 |
Life Technologies |
A-11039 |
1:500 dilution |
| Antibody |
Goat anti-mouse Alexa Fluor 568 |
Life Technologies |
A-11001 |
1:500 dilution |
| Antibody |
Goat anti-mouse Alexa Fluor 633 |
Life Technologies |
A-21050 |
1:500 dilution |
| Antibody |
Goat anti-rat Alexa Fluor 568 |
Life Technologies |
A-21050 |
1:500 dilution |
| Genetic reagent (D. melanogaster) |
unc-4DBD/FM7GFP; 20XUASCsChrimson_attp40/cyo |
Lacin et al., 2020
|
|
|
| Genetic reagent (D. melanogaster) |
unc-4AD/FM7; 20X-UASChrimson_attp40/cyo |
Lacin et al., 2020
|
|
|
| Genetic reagent (D. melanogaster) |
sens2-GAL4-DBD |
Lacin et al., 2024
|
|
|
| Genetic reagent (D. melanogaster) |
P{w[+mW.hs]=GawB}elav[C155]; P{w[+mW.hs]=FRT(w[hs])}G13 P{w[+mC]=tubP GAL80}LL2 |
Tzumin Lee Lab |
|
|
| Genetic reagent (D. melanogaster) |
20XUAS-CsChrimson-mVenus_attp18 |
V. Jayaraman lab |
|
|
| Genetic reagent (D. melanogaster) |
20XUAS >FRT-stop>CsChrimson-mVenus_attp18 |
V. Jayaraman lab |
|
|
| Genetic reagent (D. melanogaster) |
P{GawB}elav[C155], P{FRT(w[hs])}G13 P{UAS-mCD8::GFP.L}LL5 |
Tzumin Lee Lab |
|
|
| Genetic reagent (D. melanogaster) |
P{FRT(w[hs])}G13 P{tubP-GAL80}LL2 |
Tzumin Lee Lab |
|
|
| Genetic reagent (D. melanogaster) |
y[1] w1118; P{tubP-GAL80}LL9 P{FRT(w[hs])}2 A/TM3, Sb |
Tzumin Lee Lab |
|
|
| Genetic reagent (D. melanogaster) |
knot-p65.AD/CyO, weep; Dr/TM6 |
Luo Lab, Hongjie Li |
|
|
| Genetic reagent (D. melanogaster) |
pin/cyo; c15-p65.AD/TM6b |
Luo Lab, Hongjie Li |
|
|
| Genetic reagent (D. melanogaster) |
tj-vp16.AD |
Desplan Lab- David Chen |
|
|
| Genetic reagent (D. melanogaster) |
twit-p65.AD |
Stephen Goodwin |
|
|
| Genetic reagent (D. melanogaster) |
13XLexAop2-IVS-myr::GFP in attP40 |
BDSC |
RRID:BDSC32210
|
|
| Genetic reagent (D. melanogaster) |
P{hsFLP}1; P{FRT(w[hs])}G13 P{tubP-GAL80}LL2/CyO |
BDSC |
RRID:BDSC5145
|
|
| Genetic reagent (D. melanogaster) |
P{tubP-GAL80}LL10 P{neoFRT}40 A/CyO |
BDSC |
RRID:BDSC5192
|
|
| Genetic reagent (D. melanogaster) |
w[*]; l(2)*[*]/CyO; Mi{Trojan-GAL4DBD.0}ChAT[MI04508-TG4DBD.0] CG7715[MI04508-TG4DBD.0-X]/TM3, Sb[1] |
BDSC |
RRID:BDSC60318
|
|
| Genetic reagent (D. melanogaster) |
w1118; PBac{RB}Fer2e03248 |
BDSC |
RRID:BDSC26028
|
|
| Genetic reagent (D. melanogaster) |
w1118; PBac{Sp1-EGFP.S}VK00033 |
BDSC |
RRID:BDSC38669
|
|
| Genetic reagent (D. melanogaster) |
w[1118]; PBac{y[+mDint2] w[+mC]=fkh GFP.FPTB}VK00037/SM5 |
BDSC |
RRID:BDSC43951
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*]; Mi{y[+mDint2]=MIC}twit[MI06552]/(SM6a) |
BDSC |
RRID:BDSC41449
|
|
| Genetic reagent (D. melanogaster) |
w[*]; Mi{Trojan-GAL4DBD.0}Dbx[MI05316-TG4DBD.0]/TM6B, Tb[1] |
Lacin et al., 2019
|
RRID:BDSC82989
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*]; Mi{Trojan-GAL4DBD.1}Lim3[MI03817-TG4DBD.1]/(CyO) |
Lacin et al., 2019
|
RRID:BDSC82990
|
|
| Genetic reagent (D. melanogaster) |
w1118; PBac{WH}Ets21Cf03639 |
BDSC |
RRID:BDSC18678
|
|
| Genetic reagent (D. melanogaster) |
w[*]; Mi{Trojan-p65AD.2}VGlut[MI04979-Tp65AD.2]/CyO |
Lacin et al., 2019
|
RRID:BDSC82986
|
|
| Genetic reagent (D. melanogaster) |
w[*]; betaTub60D[Pin-1]/CyO; Mi{Trojan-p65AD.1}Dr[MI14348-Tp65AD.1] |
Lacin et al., 2019
|
RRID:BDSC82991
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*]; Mi{y[+mDint2]=MIC}Dr[MI14348]/TM3, Sb[1] Ser[1] |
BDSC |
RRID:BDSC59504
|
|
| Genetic reagent (D. melanogaster) |
w[*]; betaTub60D[Pin-1]/CyO; TI{2 A-GAL4(DBD)::Zip-}HGTX[DBD]/TM6B, Tb[1] |
Lacin et al., 2019
|
RRID:BDSC82992
|
|
| Genetic reagent (D. melanogaster) |
ey-GAL4-DBD |
Lacin et al., 2019
|
RRID:BDSC6294
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*]; Mi{y[+mDint2]=MIC}Ets65A[MI05707] |
BDSC |
RRID:BDSC40235
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*]; TI{GFP[3xP3.cLa]=CRIMIC.TG4.2}sv[CR00370-TG4.2] |
BDSC |
RRID:BDSC78901
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*]; TI{GFP[3xP3.cLa]=CRIMIC.TG4.2}Sox21a[CR00451-TG4.2]/TM3 Sb[1] Ser[1] |
BDSC |
RRID:BDSC83174
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*] Mi{y[+mDint2]=MIC}bi[MI08152] lncRNA:CR32773[MI08152] |
BDSC |
RRID:BDSC51220
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*]; Mi{y[+mDint2]=MIC}ap[MI01996]/CyO |
BDSC |
RRID:BDSC42297
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*]; Mi{y[+mDint2]=MIC}inv[MI09433] |
BDSC |
RRID:BDSC52163
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*] Mi{y[+mDint2]=MIC}acj6[MI07818] |
BDSC |
RRID:BDSC51212
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*]; Mi{PT-GFSTF.2}Hmx[MI02025-GFSTF.2]/TM3, Sb[1] Ser[1] |
BDSC |
RRID:BDSC59785
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*]; Mi{y[+mDint2]=MIC}Hmx[MI02896] |
BDSC |
RRID:BDSC36161
|
|
| Genetic reagent (D. melanogaster) |
y[1] w[*]; Mi{y[+mDint2]=MIC}Ets65A[MI05707] |
BDSC |
RRID:BDSC40235
|
|
| Genetic reagent (D. melanogaster) |
y[1]; Mi{y[+mDint2]=MIC}toy[MI03240] |
BDSC |
RRID:BDSC61701
|
|
| Genetic reagent (D. melanogaster) |
P{Tub-dVP16AD.D} |
BDSC |
RRID:BDSC60295
|
|
| Genetic reagent (D. melanogaster) |
P{Tub-GAL4DBD.D} |
BDSC |
RRID:BDSC0298
|
|
| Genetic reagent (D. melanogaster) |
lim3-GAL4-DBD |
Lacin et al., 2019
|
RRID:BDSC82990
|
|
| Genetic reagent (D. melanogaster) |
ChAT-p65.AD |
Lacin et al., 2019
|
|
RMCE with RRID:BDSC37817
|
| Genetic reagent (D. melanogaster) |
y[*]w[*]/w[*];inv[MI09433.p65AD_1]/SM6a |
this study |
|
RMCE with RRID:BDSC52163, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
ap-GAL4-DBD |
this study |
|
RMCE with RRID:BDSC42297, request from Lacin lab BDSC52163
|
| Genetic reagent (D. melanogaster) |
ap-p65.AD |
this study |
|
RMCE with RRID:BDSC42297, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
mab-21-GAL4-DBD |
this study |
|
RMCE with RRID:BDSC59220, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
mab-21-p65.AD |
this study |
|
RMCE with RRID:BDSC59220, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
toy-GAL4-DBD |
this study |
|
RMCE with RRID:BDSC61701, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
toy-p65.AD |
this study |
|
RMCE with RRID:BDSC61701, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
shaven-p65.AD |
this study |
|
RMCE with RRID:BDSC78901, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
sox21a-GAL4-DBD |
this study |
|
RMCE with RRID:BDSC93174, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
bi-GAL4-DBD |
this study |
|
RMCE with RRID:BDSC51220, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
bi-p65.AD |
this study |
|
RMCE with RRID:BDSC51220, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
CG4328-p65.AD |
this study |
|
RMCE with RRID:BDSC42307, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
Ets65A-GAL4-DBD
|
this study |
|
RMCE with RRID:BDSC56352, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
Hmx-GAL4-DBD |
this study |
|
RMCE with RRID:BDSC36161, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
dmrt99b-GAL4-DBD |
this study |
|
RMCE with RRID:BDSC92707, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
dmrt99b-p65.AD |
this study |
|
RMCE with RRID:BDSC92707, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
Dr-GAL4-DBD |
this study |
|
RMCE with RRID:BDSC59504, request from Lacin lab |
| Genetic reagent (D. melanogaster) |
exex-GAL4-DBD |
this study |
|
RMCE with exex-p65AD[attP2FRT2], request from Lacin lab |
| Genetic reagent (D. melanogaster) |
vg-GAL4-DBD |
this study |
|
RMCE with vg-p65AD[attP2FRT2], request from Lacin lab |
| Genetic reagent (D. melanogaster) |
sens2-p65.AD |
this study |
|
RMCE with sens2-GAL4-DBD[attP2FRT2], request from Lacin lab |
| Genetic reagent (D. melanogaster) |
Ets21C-GAL4-DBD |
this study |
|
RMCE with Ets21C-p65.AD[attP2FRT2], request from Lacin lab |
| Genetic reagent (D. melanogaster) |
eve-GAL4-DBD |
this study |
|
RMCE with eve-p65.AD[attP2FRT2], request from Lacin lab |
| Genetic reagent (D. melanogaster) |
exex-p65.AD[attP2FRT2] |
this study |
|
CRISPR /Trojan (CRIMIC), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
exex-GAL4-DBD[attP2FRT2] |
this study |
|
CRISPR /Trojan (CRIMIC), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
eve-p65.AD[attP2FRT2] |
this study |
|
CRISPR /Trojan (CRIMIC), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
vg-p65.AD[attP2FRT2] |
this study |
|
CRISPR /Trojan (CRIMIC), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
vg-GAL4-DBD[attP2FRT2] |
this study |
|
CRISPR /Trojan (CRIMIC), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
H15-p65.AD[attP2FRT2] |
this study |
|
CRISPR /Trojan (CRIMIC), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
scro-p65.AD[attP2FRT2] |
this study |
|
CRISPR /Trojan (CRIMIC), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
scro-GAL4-DBD[attP2FRT2] |
this study |
|
CRISPR /Trojan (CRIMIC), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
Ets21C-p65.AD[attP2FRT2] |
this study |
|
CRISPR /Trojan (CRIMIC), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
Ets21C-GAL4-DBD[attP2FRT2] |
this study |
|
CRISPR /Trojan (CRIMIC), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
eve-p65.AD[attP2FRT2] |
this study |
|
CRISPR /Trojan (CRIMIC), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
Fer3-GAL4-DBD |
this study |
|
CRISPR /In frame insertion (C terminus), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
ems-GAL4-DBD |
this study |
|
CRISPR /In frame insertion (C terminus), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
HLH4C-GAL4-DBD |
this study |
|
CRISPR /In frame insertion (2nd exon), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
w1118;; fkh-T2A-GAL4-DBD/TM6b |
this study |
|
CRISPR /In frame insertion (C terminus for RA isoform), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
w*;; D-VP16/TM6b |
this study |
|
CRISPR /In frame insertion (C terminus), request from Lacin lab |
| Genetic reagent (D. melanogaster) |
pJFRC29-10XUAS-IVS-myr::GFP-p10 in attP40 or attP2 |
Rubin Lab |
|
|
| Genetic reagent (D. melanogaster) |
pJFRC105-10XUAS-IVS-nlstdTomato in VK0003 |
Rubin Lab |
|
|
| Genetic reagent (D.
melanogater) |
pJFRC12-10XUAS-IVS-myr::GFP attp40 or attP2 |
Rubin Lab |
|
|
| Genetic reagent (D.
melanogater) |
pJFRC28-10XUAS-IVS-GFP-p10 in attP2 |
Rubin Lab |
|
|
| Chemical compound, drug |
Paraformaldehyde |
EMS |
15713 |
|
| Chemical compound, drug |
Vectashield |
Vectorlabs |
H-1000 |
|
| Chemical compound, drug |
DPX |
Electron Microscopy Sciences |
50980370 |
|
| Chemical compound, drug |
Gibson Assembly Master Mix |
New England Biolabs |
E2621S |
|
| Recombinant DNA reagent |
pCFD4-U6:1_U6:3tandemgRNAs |
Addgene |
49411 |
|
| Recombinant DNA reagent |
pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70 |
Addgene |
62897 |
|
| Recombinant DNA reagent |
pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70 |
Addgene |
62903 |
|
| Recombinant DNA reagent |
pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70 |
Addgene |
62914 |
|
| Recombinant DNA reagent |
pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70 |
Addgene |
62896 |
|
| Recombinant DNA reagent |
pBS-KS-attB2-SA(0)-T2A-Gal4DBD-Hsp70 |
Addgene |
62902 |
|
| Recombinant DNA reagent |
pBS-KS-attB2-SA(0)-T2A-p65AD-Hsp70 |
Addgene |
62912 |
|
| Recombinant DNA reagent |
pBS-KS-attP2FRT2-SA(0)-T2A-p65AD-Hsp70 |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pBS-KS-attP2FRT2-SA(1)-T2A-p65AD-Hsp70 |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pBS-KS-attP2FRT2-SA(2)-T2A-p65AD-Hsp70 |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pBS-KS-attP2FRT2-SA(0)-T2A-gal4DBD-Hsp70 |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pBS-KS-attP2FRT2-SA(1)-T2A-gal4DBD-Hsp70 |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pBS-KS-attP2FRT2-SA(2)-T2A-gal4DBD-Hsp70 |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pCFD4-exex |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pCFD4-vg |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pCFD4-H15 |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pUC57_Hb9 |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pUC57_vg |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pUC57_H15 |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pUC57_gw_OK2_Scro |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pUC57_gw_OK2_Ets21C |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pUC57_gw_OK2_eve |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pUC57_gw_OK2_Fer3 |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pUC57_gw_OK2_ems |
this study |
|
request from Lacin lab |
| Recombinant DNA reagent |
pUC57_gw_OK2_HLH4C |
this study |
|
request from Lacin lab |