FIG. 1.

The Tsg24 protein transiently accumulates in the centromeric regions of mitotic chromosomes. Interphase or mitotic CHO cells were fixed with methanol-acetone (50:50 [vol/vol]) and triple stained with the anti-Tsg24 antibody (1:20) or preimmune serum (1:20), a CREST antiserum (1:500), and Hoechst 33258 in an indirect immunofluorescence microscopy experiment. The secondary antibodies were a fluorescein isothiocyanate-conjugated swine anti-rabbit IgG and a rhodamine-conjugated goat anti-human IgG. The cells were analyzed by indirect immunofluorescence microscopy.