FIG. 3.

The Tsg24 protein is uniformly expressed throughout the cell cycle. Protein extracts were prepared from a serum-deprived synchronized population of CHO cells, following the addition of serum to the cells. Equal numbers of cells were sampled at the indicated time points (0 to 24 h). The extracts were analyzed by immunoblotting with the anti-Tsg24 serum. To ensure that equal amounts of proteins had been loaded onto the gel at all time points, the same immunoblot was labelled in parallel with an anti-α-lamin antibody. m, mitotic extract.