Fig. 2|.

Inactivation of the cxcA gene delays daughter cell separation and reduces lysis of cells devoid of ClpXP. (A) Schematic of the genomic and protein domain organization of CxcA. (B) Spot-dilution assay: WT and mutant strains were grown exponentially at 37 °C in TSB. Subsequently, 10-fold serially diluted cells (OD₆₀₀ ∼ 0.4) were spotted on TSA-agar plates that were incubated at 25 °C or 37 °C for respectively 48 and 24 h. (C) Growth of single colonies were studied by time-lapse microscopy at 25 °C: Images were acquired every 5 min for at least 48 h. (D) Daughter cell splitting was tracked by labeling with HADA (blue, label PG synthesis) and WGA-488 (yellow; binds to the outer cell wall but not the septum) before imaging with SR-SIM, a scale-bar of 2 μm is on the overlay image. For each strain, the proportion of cells that proceeded to daughter cell split, was quantified for at least 500 cells displaying a closed HADA-stained septum. Statistical analysis was performed using the chi square test for independence. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)