TABLE 1.
Differentiation profile of 32D cells overexpressing c-myb and B-myba
| Cell lineb | Days after G-CSF addition | % of following cell types
|
||
|---|---|---|---|---|
| Myeloblast | Promyelocyte/myelocyte | Metamyelocyte/granulocyte | ||
| 32D/PMT-Neo (−Zn) | 0 | 97.7 ± 1.5 | 2.3 ± 1.5 | |
| 4 | 7.0 ± 1 | 85.7 ± 1.5 | 7.3 ± 0.6 | |
| 7 | 0.3 ± 0.6 | 76.0 ± 4.6 | 23.3 ± 2.5 | |
| 10 | 1.3 ± 1.5 | 98.7 ± 5.3 | ||
| 32D/PMT-Neo (+Zn) | 0 | 98.3 ± 0.6 | 1.7 ± 0.6 | |
| 4 | 12.0 ± 3 | 77.7 ± 6.8 | 10.3 ± 4 | |
| 7 | 0.7 ± 0.6 | 80.3 ± 2.5 | 19.0 ± 2.6 | |
| 10 | 3.3 ± 3.5 | 96.7 ± 10.1 | ||
| 32D/c-myb (−Zn) | 0 | 97.7 ± 0.7 | 2.3 ± 0.6 | |
| 4 | 10.7 ± 1.2 | 86.3 ± 1.5 | 3.0 ± 1 | |
| 7 | 1.0 ± 1.7 | 83.0 ± 1.5 | 15.4 ± 2.5 | |
| 10 | 66.7 ± 5.7 | 33.3 ± 10.8 | ||
| 32D/c-myb (+Zn) | 0 | 99.0 ± 1 | 1.0 ± 1 | |
| 4 | 23.0 ± 8.7 | 75.0 ± 7.8 | 2.0 ± 1 | |
| 7 | 2.7 ± 2.5 | 95.0 ± 3 | 2.3 ± 0.6 | |
| 10 | 10.0 ± 2 | 87.3 ± 3.2 | 2.7 ± 1.5 | |
| 32D/B-myb (−Zn) | 0 | 95.7 ± 2.5 | 4.3 ± 2.5 | |
| 4 | 1.7 ± 1.2 | 50.0 ± 1 | 48.3 ± 5.6 | |
| 7 | 6.7 ± 3.2 | 93.3 ± 8.5 | ||
a
32D cells overexpressing each transgene were induced to differentiate by the addition of G-CSF. On the indicated days, aliquots of the cells were cytospun and stained with May-Grunwald-Giemsa. The values are means of percentages of the indicated cells from three independent studies ± standard deviations.
b
−Zn, lacking zinc; +Zn, with zinc.