TABLE 2.
Differentiation profiles of 32D cells overexpressing chimeric genes between c-myb and B-myba
| Cell line | Days after GCSF addition | % of following cell type (mean ± SD)
|
||
|---|---|---|---|---|
| Myeloblast | Promyelocyte/myelocyte | Metamyelocyte/granulocyte | ||
| 32D/PMT-Neo (−Zn) | 0 | 97.7 ± 1.5 | 2.3 ± 1.5 | |
| 4 | 7.0 ± 1 | 85.7 ± 1.5 | 7.3 ± 0.6 | |
| 7 | 0.3 ± 0.6 | 76.0 ± 4.6 | 23.3 ± 2.5 | |
| 32D/PMT-Neo (+Zn) | 0 | 98.3 ± 0.6 | 1.7 ± 0.6 | |
| 4 | 12.0 ± 3 | 77.7 ± 6.8 | 10.3 ± 4 | |
| 7 | 0.7 ± 0.6 | 80.3 ± 2.5 | 19.0 ± 2.6 | |
| Chimera CBC (−Zn) | 0 | 99.0 ± 1 | 1.0 ± 1 | |
| 4 | 3.7 ± 1.2 | 85.0 ± 3.6 | 11.3 ± 2.5 | |
| 7 | 0.7 ± 1.1 | 77.7 ± 4.6 | 21.3 ± 3.8 | |
| Chimera CBC (+Zn) | 0 | 97.7 ± 1.5 | 2.3 ± 1.5 | |
| 4 | 2.7 ± 2 | 84.0 ± 1 | 13.3 ± 2.3 | |
| 7 | 74.0 ± 7.8 | 26.0 ± 6.5 | ||
| Chimera CCB (−Zn) | 0 | 98.0 ± 1 | 2.0 ± 1 | |
| 4 | 47.3 ± 2.5 | 52.7 ± 2.9 | ||
| 7 | 2.7 ± 2.5 | 97.3 ± 8.7 | ||
| Chimera CCB (+Zn) | 0 | 97 ± 1 | 3.0 ± 1 | |
| 4 | 44.0 ± 3.6 | 56.0 ± 6 | ||
| 7 | 2.7 ± 2.5 | 97.3 ± 7.5 | ||
| Chimera CBB (−Zn) | 0 | 97 ± 1 | 3.0 ± 1 | |
| 4 | 34.7 ± 2.5 | 65.3 ± 4.9 | ||
| 7 | 1.0 ± 1 | 99.0 ± 3.4 | ||
| Chimera CBB (+Zn) | 0 | 97.7 ± 1.5 | 2.3 ± 1.5 | |
| 4 | 10.3 ± 2.3 | 89.7 ± 6.5 | ||
| 7 | 3.0 ± 1 | 97.0 ± 3.6 | ||
a
32D cells overexpressing each transgene were induced to differentiate by G-CSF. On the indicated days, aliquots of the cells were cytospun and stained with May-Grunwald-Giemsa. The values are means from three studies ± standard deviations.
b
−Zn, lacking zinc; +Zn, with zinc.