FIG. 2.

AHR dose-dependent reporter gene expression in CV-1 (A) and Hepa-1 (B) cells. Transient transfections contained fixed amounts of pAhRDSV40Luc3 reporter gene, pCMVβgal, and pcDNAIneo/mARNT, and increasing amounts of pcDNAI/B6AHR, as shown on the abscissa. Twenty-four hours after transfection, cells were treated with DMSO vehicle control or 10 nM TCDD. Luciferase and β-galactosidase activities were measured 14 to 16 h after treatment. The ordinate represents luciferase activity normalized to β-galactosidase activity.